Compliance of functional exercise and its influencing factors among patients with Parkinson's disease / 预防医学

Objective@# To explore the compliance of functional exercise and related factors in patients with Parkinson's disease（PD）. @*Methods@# A total of 108 PD patients were selected from Department of Neurology of General Hospital of Panzhihua Iron and Steel Group. An anonymous survey was conducted with general questionnaire,Mini-Mental State Examination,Hamilton Depression Rating Scale,Hamilton Anxiety Rating Scale and functional exercise compliance questionnaire. Multivariate linear regression model was used to analyze the influencing factors for the scores of functional exercise compliance in PD patients. @*Results@# The total score of functional exercise compliance of 108 PD patients was 36.88±4.62. The scores of compliance of physical exercise,exercise monitoring,seeking advice and tips for attention were 15.65±1.92,9.61±1.48,5.67±1.08 and 5.95±1.18,respectively. The results of multivariate linear regression analysis showed that educational background（β'=0.748）,duration of illness（β'=-0.252）,Hoehn-Yahr stage（β'=-0.677）,rehabilitation guidance（β'=0.293）,self-care ability（β'=0.313）,primary caregiver（β'=0.182）,cognitive dysfunction（β'=0.745）,depression（β'=0.588）and anxiety（β'=0.520）could affect the compliance of functional exercise among PD patients（all P<0.05）. @*Conclusion @#The compliance of functional exercise among PD patients was at low level,with educational background,duration of illness,Hoehn-Yahr stage,rehabilitation guidance,self-care ability,primary caregiver,cognitive dysfunction,depression and anxiety as the influencing factors.

rBMSCs/ITGA5B1 Promotes Human Vascular Smooth Muscle Cell Differentiation via Enhancing Nitric Oxide Production

BACKGROUND AND OBJECTIVES: Previous studies have shown that integrins alpha5beta1 (ITGA5B1) gene-modified rat bone marrow mesenchymal stem cells (rBMSCs) could prevent cell anoikis and increase the nitric oxide (NO) production. Here we examined the capability of rBMSCs/ITGA5B1 on the phenotype modulation of Human Pulmonary Artery Smooth Muscle Cell (HPASMC) in vitro. METHODS AND RESULTS: The synthetic (dedifferentiated) phenotype of HPASMC was induced by monocrotaline (MCT, 1μM) for 24 h and then co-cultured with rBMSCs/ITGA5B1 in a transwell culture system. The activation of NO/cGMP (nitric oxide/Guanosine-3′, 5′-cyclic monophosphate) signaling was investigated in HPASMC. The changes of pro-inflammatory factors, oxidative stress, vasodilator, vasoconstrictor, contractile and synthetic genes, and the morphological changes of HPASMC were investigated. The results of this study showed that the NO/cGMP signal, endothelial nitric oxide synthase (eNOS) expression, the expression of the vasoprotective genes heme oxygenase-1 (HMOX1) and prostaglandin-endoperoxide synthase 2 (PTGS2) were increased, but the expression of transforming growth factor-β1 (TGF-β1), CCAAT/enhancer-binding proteins delta (Cebpd), Krüppel-like factor 4 (KLF4), and activating transcription factor 4 (ATF4) were reduced in MCT treated HPASMC co-cultured with rBMSCs/ITGA5B1. The synthetic smooth muscle cells (SMCs) phenotype markers thrombospondin-1, epiregulin and the vasoconstrictor endothelin (ET)-1, thromboxane A2 receptor (TbxA2R) were down-regulated, whereas the contractile SMCs phenotype marker transgelin expression was up-regulated by rBMSCs/ITGA5B1. Furthermore, rBMSCs/ITGA5B1 promoted the morphological restoration from synthetic (dedifferentiation) to contractile (differentiation) phenotype in MCT treated HPASMC. CONCLUSIONS: rBMSCs/ITGA5B1 could inhibit inflammation and oxidative stress related genes to promote the HPASMC cell differentiation by activation NO/cGMP signal.

Effect of Budesonide combined with clarithromycin on chronic rhinosinusitisandits mucosa remodeling / 中国耳鼻咽喉头颈外科

OBJECTIVE To explore the application of Budesonide combined with clarithromycin in the treatment of patients with chronic rhinosinusitis(CRS) and observe the effect on mucosa tissue remodeling.METHODS 90 cases of CRS patients were selected in our hospital.They were randomly divided into Budesonide treatment group,clarithromycin treatment group and the two combined treatment group.VAS score,sinus CT Score were compared among the three groups before and after treatment.Airway remodeling and the occurrence of adverse reactions were also compared among the three groups.RESULTS After treatment,VAS score and Sinus CT scoreof the combined treatment groupwere significantly better than that of the other two groups(P＜0.05);Airway damage in combined treatment group was also lighter,basement membrane thickening was not obvious.The gray value of TGF-β1 in the combined treatment group was significantly higher than that of the other two groups(P＜0.05);Drug adverse reaction occurred less and resolved after treatment.CONCLUSION Budesonidc combined with clarithromycin can significantly improve the state of illness in patients with CRS and alleviate mucosal tissue remodeling.It is worthy of recommendation in clinical practice.

Effect of traditional Chinese medicine composit Guben Yiliu III combined with gemcitabine on human pancreatic cancer xenograft in nude mice / 中国实验动物学报

Objective To explore whether the Chinese medicine Guben Yiliu III can improve the effect of gemcit -abine on human pancreatic cancer xenograft in nude mice . Methods Nude mice with transplanted human pancreatic cancer were divided randomly into 4 groups: control group, gemcitabine treatment group , combined ( Guben Yiliu III +gemcitabine) group, and Guben Yiliu III group, 10 mice in each group.The gemcitabine group and combined group were treated with gemcitabine from the 8th day after transplantation in a dose of 100 mg/kg by i.p.injection, twice a week. Guben Yiliu III and combined groups were given the aqueous solution of Guben Yiliu III granules p .o.since the 8th day af-ter transplantation .Result The inhibition rate of transplanted tumor in the three treatment groups were 48.9% in the gemcitabine group , 68.9%in the combined group , and 28.0%in the Guben Yiliu III group .The combined group showed a significantly higher inhibition rate than the gemcitabine group (P<0.05).The gemcitabine group, combined group and Guben Yiliu III group showed a significantly slower growth rate than the control group .However, the combined treatment group showed a pronounced side effect and body weight loss than the other 3 groups .Conclusions The Chinese medicine Guben Yiliu III can improve the inhibitory effect of gemcitabine on nude mice with human pancreatic cancer xenograft in the auxilla of nude mice .

Roles of apoptosis in normal and tumor microenvironment / 国际肿瘤学杂志

Apoptosis is the autonomous death of cells,which is controlled by the genes and to maintain the homeostasis of the body.Apoptosis is the important mechanism in the cell's growth and development.Recent studies have found that apoptosis can affect the neighboring cells physiological process in microenvironment through directly contact or release a series of regulatory factor,which plays an important roles in the construction of tumor microenvironment.Therefore,understanding the roles of apoptosis in the normal and tumor microenvironment has great significance for further study the pathogenesis,prevention and treatment of tumor.

Enhanced SEC2 mutants and their superantigen activities / 生物工程学报

As a superantigen protein, Staphylococcal enterotoxin C2 (SEC2) activates the immune system effectively even in extremely low concentrations, and this property could be applied in adjuvant therapy against tumors and infectious diseases. In order to enhance the superantigen activity of SEC2, the residues at position 102-106 of native SEC2 were substituted for WWH, WWT and WWP by over-lap PCR, and three mutants named ST-1, ST-2 and ST-3 were obtained. Stimulating activity to murine lymphocytes proliferation and inhibiting activity to tumor cell growth of the three mutants were significantly improved compared with the native SEC2. Febrile activities of ST-1 and ST-3 were comparable with the native SEC2, but ST-2 showed markedly increased febrile activity than native SEC2. Moreover, the levels of IL-2, IFN-gamma and TNF-alpha secreted by T cells stimulated with the three mutants were significantly improved, which might be the possible reason for enhanced tumor cell growth inhibition activities. Furthermore, mVbeta8.2 gene transcription levels of murine splenocytes stimulated by the three mutants were dramatically increased compared with native SEC2, suggesting their increased affinities to TCR mVbeta8.2 molecular, which might be the main reason for their enhanced superantigen activities.

ECG signal detecting and processing system based on LabVIEW / 医疗卫生装备

A system of detection for ECG signal based on virtual instrument and LabVIEW is introduced.The conception of virtual instrument,ECG amplifier data acquisition(DAQ) and the data procession software are expounded.The system has the function of real-time displaying the ECG,data acquisition and Heart Rate Variability(HRV) analysis.

Constructing a Cox proportional hazard regression model of prognosis factors of acute myocardial infarction by retrospective cohort study / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effects of the potential factors, including Chinese herbal decoction, on the long-term prognosis of acute myocardial infarction (AMI).</p><p><b>METHODS</b>Previous clinical data of 162 patients with AMI were collected, who were followed-up to observe the important events for prognosis, as death and cardio-cerebral episode, and the Cox proportional hazards regression model was used to assess the relative factors.</p><p><b>RESULTS</b>The degree of cardiac function (by New York grading) increased 1 grade when age increased for 10 years, and the relative hazardous degree (RHD) raised to 1.983 and 3.169. After treatment with Chinese herbal decoction and angiotensin converting enzyme inhibitor (ACEI), the RHD could be reduced to 0.177 and 0.161 respectively. Taking the important cardio-cerebral events, including death, as the endpoint, when age increased for 10 years, the cardiac function would increase for 1 grade and RHD of endpoint events increased to 2.021 and 1.863, if patients had history of anterior infarction, arrhythmia and diabetes mellitus, it increased to 2.903, 2.588 and 4.039 respectively. Chinese decoction and ACEI treatment could reduce it to 0.093 and 0.141 respectively.</p><p><b>CONCLUSION</b>Age, heart failure, anterior infarction, arrhythmia and diabetes mellitus are the hazardous factors of the long-term prognosis of AMI, Chinese herbal decoction and ACEI are the protective factors.</p>

Diagnostic value of B-type natriuretic peptide in pati ents with acute dyspnea / 中华全科医师杂志

Objective To determine the si gnificance of plasma level of B-type natriuretic peptide (BNP) in patients with congestive heart failure (CHF) for differential diagnosis of their acute dyspne aMethods Totally, 130 patients with acute dyspnea were selected, including 78 patients diagnosed as CHF with left ventricular ejection fraction less than 040 and 52 patients without CHF Plasma level of BNP was m easured by a rapid, point-of-care quantitative immunofluorescent assay Results Plasma level of BNP was(732?53)ng/L in patients wit h CHF, much higher than that in those without CHF(71?34)ng/L(P

Effect of Early Treatment of Chinese Herbal Medicine on Long-term Prognosis of Acute Myocardial Infarction / 广州中医药大学学报

[Objective] To explore the effect of early treatment of Chinese herbal medicine on the long-term prognosis of acute myocardial infarction (AMI) . [Methods] One hundred and fifty-seven AMI patients were divided into two groups according to the treatment during the hospitalization: 129 patients treated with Chinese herbal medicine and western medicine were in group A, and 28 patients treated with western medicine only were in group B. Statistical analysis of age, sex, infarction location, complications and medical history was made in all of the patients. A follow-up survey was made to investigate the subsistence of the patients and the incidences of all the events (including death and severe cardio-cerebrovascular events) . [Results] With the death as the end event, the survival graph of group A was higher than group B (P=0.1166); when with all the severe events as the end event, the survival graph of group A was still higher ( P=0.048) .[Conclusion] The probability of incidences of severe events including death in group A is lower than that in group B.